Kamala Hospital and Research Center for Thalassemia and Sickle Cell patients

Kamala Hospital and Research Laboratory was established (supported by Prema Bai Dakotiya Charitable and Memorial Society) with an aim to screen the patients for various mutations in alpha, beta, gamma genes causing thalassemia. The main objective of the research is to conduct various studies to improve the quality of life of the patient and to formulate better treatment strategies

Main aims of molecular  research lab

  • To maintain a demographic, clinical database of all the patients which would help in formulating better treatment profiles
  • To extract DNA from the patients and maintain a DNA databank. The DNA would be screened for mutations in different hemoglobin genes to confirm their diagnosis.
  • To develop rapid screening methods for screening different haemoglobinopathies
  • To study the impact of repeated blood transfusions on quality of life of thalassemia patients
  • To study the effect of modifier genes on the severity of thalassemia

National and International Collaborations

  • Institute of Genomics and Integrated Biology (IGIB), New Delhi for working on gene therapy for Thalassemia and Sickle Cell Anemia
  • Centre for DNA Fingerprinting and Diagnostics for prenatal diagnosis
  • Aurelia Maggio, Dept of Hematology and Rare Diseases, Palermo, Italy to be a part of "Health Repository Data for developing an International Prognostic Scoring System for Thalassemia”
  • Chrogene Aarogyam Biotech Private Limited for developing Noninvasive device for detecting Sickle Cell Anemia
  • VNR Vignana Jyothi Institute of Engineering and Technology, Hyderabad for Identification of Sickle Cell Anemia using Deep Neural Networks through Artificial Intelligence
  • Collaborated with Novartis in SWAY SURVEY and contributed data on 123 patients reflecting the Indian scenario at the international level

Human Resources:

S. No Name of the Person Designation Status of employment (Part / full time)
1 Dr. Suman Jain Chief Medical Research Officer & Secretary Full Time
2 Dr.G.Padma Research Scientist Full Time
3 Dr.M.Mamatha Research Scientist Full Time
4 Dr.A.Sudheer Research Scientist Full time
5 Dr. Sujai Suneetha Research Technical Advisor Part Time
6 Dr. V R Rao Research Advisor Consultant
7 Dr. Anjana Munshi Research Advisor Consultant
8 Dr. K. Saroja Medical Officer Full Time
9 Mr.Vamsi Kumar Technician Full Time
10 Mr. Jaleel Technician Full Time
11 Mr. Chandra Technician Full Time
12 Mr. Bharat Technician Full Time
13 Mr. Taufeeq Baig Technician Full Time
Ongoing Research Projects:  7
Projects Submitted for Funding and Under Review: 1 and 1 project is shortlisted by DBT
Research Papers Published (Thalassemia and Sickle Cell Anemia): 16

LIST OF  Projects

Ongoing

  • Ascertaining Serum Ferritin Levels in comparison with MRI in the clinical management of Beta Thalassemia Major. Intramural. One year. Rs.10,00,000/-
  • Screening of Antenatal Pregnant women for Thalassemia and Sickle Cell Anaemia as a step towards awareness and prevention. Intramural. One year. Rs. 20,12,750/-

Completed

  • Noninvasive Point of care diagnostics for Sickle Cell Disease. Department of Biotechnology (DBT), GoI under DIRAC, Big Grant. 18 months. Rs. 4984000/-
  • β -Thalassemia Disease Burden Mutation Micro ᵝ Profiling in Populations of Telangana. Telangana State Council of Science and Technology (TCOST) under Assistance for Development of State S & T Councils Programme of DST (GOI). One year. Rs.6,78,000/-
  • Amelioration of the severity of Beta thalassemia by modifier genes. Department of Biotechnology (DBT), GoI. Three years. Rs. 51,68,000/-
  • Precise Gene Correction of the Hemophilia B and Beta thalassemia disease mutations in Human Induced Pluripotent Stem Cells (hiPSCs) Using Gene Editing Nucleases for Cell-Based Therapy Duration in collaboration with Institute of Genomics and Integrative Biology (CSIR-IGIB
  • Sickle cell anaemia mission” (Genome editing and stem cell research approach for sickle cell anaemia) in collaboration with Institute of Genomics and Integrative Biology (CSIR-IGIB)

Submitted

  • Screening of antenatal women for hemoglobinopathies and newborns for metabolic genetic disorders in Jayashankar Bhupalpalli district, Telangana. Submitted to Department of Biotechnology under UMMID initiative. Three years. Rs. 3,00,25,380/-
  • Newborn Screening for Sickle Cell Disease: A Pilot study in Telangana Population. SERB under Core Research Grant. Three Years. Rs. 6992980/-
  • Influence of modifier genes and regulatory elements of β-globin gene on phenotypic expression of Thalassemia Intermedia. SERB under Start Up Research Grant. Two years. Rs.25,96,000/-

LIST OF  PUBLICATIONS ON THALASSEMIA

Papers Published
  • Osunkwo I, Andemariam B, Minniti CP, Inusa BPD, El Rassi F, Francis-Gibson B, Nero A, Trimnell C, Abboud MR, Arlet JB, Colombatti R, de Montalembert M, Jain S, Jastaniah W, Nur E, Pita M, DeBonnett L, Ramscar N, Bailey T, Rajkovic-Hooley O, James J. Impact of sickle cell disease on patients' daily lives, symptoms reported, and disease management strategies: Results from the international Sickle Cell World Assessment Survey (SWAY). Am J Hematol, 2021; 96(4):404-417
  • Priya Thakur, Nupur Bhargava, Shashank Jaitly, Pragya Gupta, Saurabh Kumar Bhattacharya, G. Padma, Saroja Kondaveeti, Suman Jain, Sivaprakash Ramalingam. Establishment and characterization of induced pluripotent stem cell line (IGIBi002-A) from a β-thalassemia patient with IVS1-5 mutation by non-integrating reprogramming approach. Stem Cell Research, 2021; 50:102124
  • Vadlamudi R. Rao, Gaurav Gupta, Kondaveeti Saroja, Suman Jain. Identification and Development of a High-Risk District Model in the Prevention of β-Thalassemia in Telangana State, India. Hemaglobin, 2020; 44(5):371-375
  • Abhinav Jain, Rahul C Bhoyar, Kavita Pandhare, Anushree Mishra, Disha Sharma, Mohamed Imran, Vigneshwar Senthivel, Mohit Kumar Divakar, Mercy Rophina, Bani Jolly, Arushi Batra, Sumit Sharma, Sanjay Siwach, Arun G Jadhao, Nikhil V Palande, Ganga Nath Jha, Nishat Ashrafi, Prashant Kumar Mishra, Vidhya A. K., Suman Jain, Debasis Dash, Nachimuthu Senthil Kumar, Andrew Vanlallawma, Ranjan Jyoti Sarma, Lalchhandama Chhakchhuak, Shantaraman Kalyanaraman, Radha Mahadevan, Sunitha Kandasamy, Pabitha B. M., Raskin Erusan Rajagopal, Ezhil Ramya J., Nirmala Devi P., Anjali Bajaj, Vishu Gupta, Samatha Mathew, SangamGoswami, Mohit,Mangla, Savinitha Prakash, Kandarp Joshi, Meyakumla, Sreedevi S., Devarshi Gajjar, Ronibala Soraisham, Rohit Yadav, Yumnam Silla Devi, Aayush Gupta, Mitali Mukerji, Sivaprakash Ramalingam, Binukumar B. K., Vinod Scaria, Sridhar Sivasubbu. IndiGenomes: a comprehensive resource of genetic variants from over 1000 Indian genomes. Nucleic Acids Research, 2021;49(D1): D1225-D1232
  • James, John & Andemariam, Biree & Inusa, Baba & El-Rassi, Fuad & Francis-Gibson, Beverly & Nero, Alecia & Minniti, Caterina & Trimnell, Cassandra & Abboud, Miguel & Arlet, Jean-Benoît & Colombatti, Raffaella & Montalembert, Mariane & Jain, Suman & Jastaniah, Wasil & Nur, Erfan & DeBonnett, Laurie & Osunkwo, Ifeyinwa. Management Strategies and Satisfaction Levels in Patients with Sickle Cell Disease: Interim Results from the International Sickle Cell World Assessment Survey (SWAY). Blood, 2019; 134(Supplement-1):1017
  • Bhargava Nupur, Jaitly Shashank, Goswami Sangam, Jain Suman, Chakraborty, Debojyoti, Ramalingam Sivaprakash. Generation and characterization of induced pluripotent stem cell line (IGIBi001-A) from a sickle cell anemia patient with homozygous β-globin mutation. Stem Cell Research, 2019; 39:101484
  • Sundaram Acharya, Arpit Mishra, Deepanjan Paul, Asgar Hussain Ansari, Mohd. Azhar, Manoj Kumar, Riya Rauthan, Namrata Sharma, Meghali Aich, Dipanjali Sinha, Saumya Sharma, Shivani Jain, Arjun Ray, Suman Jain, Sivaprakash Ramalingam, Souvik Maiti, Debojyoti Chakraborty. Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing. Proceedings of the National Academy of Sciences, 2019; 116(42):20959-20968
  • Agarwal RK, Sedai A, Ankita K, Parmar L, Dhanya R, Dhimal S, Sriniwas R, Gowda A, Gujjal P, H P, Jain S, Ramaiah JD, Jali S, Tallur NR, Ramprakash S, Faulkner L. Information Technology-Assisted Treatment Planning and Performance Assessment for Severe Thalassemia Care in Low- and Middle-Income Countries: Observational Study. JMIR Med Inform, 2019; 7(1): e9291
  • Gayatri Rangarajan Iyer, Aruna Priya Kamireddy, Saroja Kondaveeti, Suman Jain, Qurratulain Hasan. Establishing Mutational Spectrum of Beta Thalassemia by Molecular Screening in a Low Resource Setting – Implications in Counseling and Prevention. International Journal of Public Health and Health Systems, 2019; 4(2): 36-43
  • Parmar L, Sedai A, Ankita K, Dhanya R, Agarwal RK, Dhimal S, Shriniwas R, Iyer HV, Gowda A, Gujjal P, Pushpa H, Jain S, Kondaveeti S, Dasaratha Ramaiah J, Raviteja, Jali S, Tallur NR, Ramprakash S, Faulkner L. Can inequity in healthcare be bridged in LMICs Multicentre experience from thalassemia day care centres in India. Pediatric Hematology Oncology Journal, 2017; 2:88-93
  • Rajat Kumar Agarwal, Amit Sedai, Kumari Ankita, Lalith Parmar, Rakesh Dhanya, Sunil Dhimal, Dr Reshma Shriniwas, Dr Sumithra P, Dr Hemanth V Iyer, Ashwini Gowda, Dr Pooja Gujjal, Dr Pradeep R, Pushpa H, Dr Suman Jain, Dr Saroja Kondaveeti, Dr J. Dasaratha Ramaiah, Dr Raviteja, Dr Hariharanatha Sharma, Dr Sujata Jali, Shrikant Viragi, Dr Shreedevi Bobati, Neelavva Rayappa Tallur, Dr Stalin Ramprakash, Dr Lawrence Faulkner, Multi-institutional, retrospective review of blood transfusion practices and outcomes in a large cohort of thalassemia patients in South India, Pediatric Hematology Oncology Journal, Available online 5 December 2017, ISSN 2468-1245, https://doi.org/10.1016/j.phoj.2017.12.001
  • Agarwal RK, Sedai A, Ankita K, Parmar L, Dhanya R, Dhimal S, Shriniwas R, Sumithra P, Hemanth V Iyer, Gowda A, Gujjal P, Pradeep R, Pushpa H, Jain S, Kondaveeti S, Dasaratha Ramaiah J, Raviteja, Sharma H, Jali S, Viragi S, Bobati S, Tallur NR, Ramprakash S, Faulkner L, Multiinstitutional, retrospective review of blood transfusion practices and outcomes in a large cohort of thalassemia patients in South India. Pediatric Hematology Oncology Journal, 2017; 2(4): 74-78
  • Dadheech S, Madhulatha D, Jain S, Joseph J, Jyothy A, Munshi A. Association of BCL11A genetic variant (rs11886868) with severity in beta thalassaemia major & sickle cell anaemia. Indian J Med Res, 2016; 143(4):449-54
  • Dadheech S, Jain S, Madhulatha D, Sharma V, Joseph J, Jyothy A, Munshi A. Association of Xmn1 -158 γG variant with severity and HbF levels in β-thalassemia major and sickle cell anaemia. Mol Biol Rep, 2014; 41(5):3331-7
  • Dadheech S, Rao AV, Shaheen U, Hussien MD, Jain S, Jyothy A, Munshi A. Three most common nonsynonymous UGT1A6*2 polymorphisms (Thr181Ala, Arg184Ser and Ser7Ala) and therapeutic response to deferiprone in β-thalassemia major patients. Gene, 2013; 531(2):301-5
  • Munshi A, Dadheech S, Jain S, Joseph J, Al-Hazzani A, Alshatwi AA, Sai Babu M, Rajeshwar K, Jyothy A. Lack of association of G779A ZHX-2 gene variant with HbF levels in β-thalassemia major. Eur J Haematol, 2011; 86(6):502-6
  • Angalena R, Prabitha KN, Chaudhary AK, Bashyam MD, Jain S, Dalal AB. A novel homozygous point mutation at codon 82 (HBB: c.247A > T) in the beta-globin gene leads to thalassemia major. Int J Lab Hematol, 2010; 32(5):548-9